Preservation and computer-aided microscopic analysis of planktonic Protozoa and algae |
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Authors: | Milos Legner W. Gary Sprules |
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Affiliation: | (1) Department of Zoology, University of Toronto, Erindale Campus, L5L 1C6 Mississauga, Ontario, Canada |
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Abstract: | Samples of microplankton and larger nanoplankton (5 to 200 m) are preserved with a combination of Lugol's solution and DaFano's fixative. Organisms are then settled on a gelatin-coated slide, dried and embedded in 40 percent glycerin. Counting and sizing is performed under a microscope using a drawing tube, which facilitates measuring the organisms with a microcomputer-interfaced caliper. An interactive computer program, written in BASIC, allows for estimating the volumes of cells in up to 40 shape/species categories. The program then saves data on a disk, retrieves them, and calculates the results either for individual species (abundance, biomass, and mean cell volume) or as a pooled size spectrum of all organisms measured. |
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Keywords: | preservation electronic caliper biomass estimation settling chambers Protozoa phytoplankton biomass size spectrum |
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