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红假单胞菌去除养鱼废水三态氮及COD的研究
引用本文:郑卉,张德民,王一农,戴海平. 红假单胞菌去除养鱼废水三态氮及COD的研究[J]. 生态科学, 2012, 31(4): 435-440
作者姓名:郑卉  张德民  王一农  戴海平
作者单位:1. 宁波大学, 应用海洋生物技术教育部重点实验室, 宁波315211; 2. 浙江省宁波市鄞州区渔业技术管理服务站, 宁波315012
基金项目:教育部科学技术研究重点项目(208053);浙江省自然科学基金人才培养项目(R305333)
摘    要:利用红假单胞菌(Rhodopseudomonas sp.)wps对鲫鱼养殖废水三态氮及COD进行处理.菌株wps对水体亚硝氮的去除率随菌浓的降低而降低,当添加菌液量降到0.4 mL(终浓度约为5×104cell·mL-1)时,其对亚硝氮的去除效果已不明显,但同时投加400μg碳源明显提高其亚硝氮去除率,高出对照20.06%;菌株wps对氨氮也有一定去除效果,但碳源的添加对氨氮的去除影响更大;同时施加0.4 mL wps菌液和40μg碳源对硝氮和COD的去除率分别比对照提高20.51%和22.03%.光照与否对其处理养鱼废水的效果影响不大.PCR-DGGE结果分析显示,菌株wps在与土著光合细菌的竞争中处于劣势.

关 键 词:红假单胞菌  鲫鱼废水  氨氮  亚硝氮  DGGE  
收稿时间:2012-08-25

Removal of inorganic nitrogen and COD from carp farming waste water by Rhodopseudomonas
ZHANG Hui,ZHANG De-min,WANG Yi-nong,DAI Hai-ping. Removal of inorganic nitrogen and COD from carp farming waste water by Rhodopseudomonas[J]. Ecologic Science, 2012, 31(4): 435-440
Authors:ZHANG Hui  ZHANG De-min  WANG Yi-nong  DAI Hai-ping
Affiliation:1.Key Laboratory of Applied Marine Biotechnology,Ningbo University,Ministry of Education,Ningbo 315211,China 2.Fishery Administration and Tech-service Station of Yinzhou,Ningbo 315012,China
Abstract:In this paper,we investigated the removal of inorganic nitrogen in carp farming waste water by Rhodopseudomonas sp. wps.The NO2--N removal ratio decreased with the decrease of concentration of strain wps.The removal ratio was unaffected at about 5×104 cell/mL of strain wps,but it was increased apparently when carbon source was added simultaneously(at a final concentration of 10 ppm),with 20.06% higher than control.The NH4+-N removal was improved by addition of strain wps,but it was removed more significantly by the carbon source addition.The addition of 5×104 cell/mL wps and 10 ppm carbon made the removal ratios of NO2-N and COD increased by 20.51% and 22.03% than the control,respectively.Light had little effect on the waste water. PCR-DGGE analysis showed that strain wps had a competitive disadvantage over indigenous photosynthetic bacteria.
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