| 急性肺损伤大鼠肺组织TOLL样受体4及CD14mRNA的表达 |
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| 引用本文： | 杨爱东,李文雯,王利霞,汪东颖,吴中华,苏中昊,庞慧芳.急性肺损伤大鼠肺组织TOLL样受体4及CD14mRNA的表达[J].中国实验动物学报,2011,19(3):216-219,278. |
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| 作者姓名： | 杨爱东 李文雯 王利霞 汪东颖 吴中华 苏中昊 庞慧芳 |
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| 作者单位： | 上海中医药大学,上海,201203 |
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| 基金项目： | 国家中医药管理局重点学科项目，上海市重点学科建设项目，上海市教委高校高水平特色发展项目，上海市高等学校科学技术发展基金项目，上海中医药大学名师研究室项目，上海高校选拔培养优秀青年教师科研专项基金 |
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| 摘 要： | 目的 探讨内毒素致急性肺损伤大鼠肺组织TOLL样受体4及CD14 mRNA表达的变化.方法 24只雄性Wistar大鼠随机分为2组:对照组、LPS模型组,每组再分为4 h和8 h两个亚组.尾静脉注射脂多糖(LPS)(10 mg/kg)建立大鼠急性肺损伤模型.检测大鼠动脉血气、肺体指数,实时荧光定量PCR测定肺组织TOLL样受体4及CD14 mRNA的表达,并观察肺组织病理变化.结果 与对照组相比,模型组4 h和8 h时大鼠肺组织中的TLR4及CD14 mRNA表达均显著增高(P＜0.05或P＜0.01).病理学观察显示,模型组大鼠肺组织出现出血及坏死.结论 内毒素致急性肺损伤的发病机制可能与TLR4及CD14 mRNA的表达升高有关.
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| 关 键 词： | 脂多糖 急性肺损伤 TOLL样受体4 |
Expression of TLR4 and CD14 mRNA in rats with acute lung injury induced by lipopolysaccharide |
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| YANG Ai-dong,LI Wen-wen,WANG Li-xia,WANG Dong-ying,WU Zhong-hua,SU Zhong-hao,Pang Hui-fang.Expression of TLR4 and CD14 mRNA in rats with acute lung injury induced by lipopolysaccharide[J].Acta Laboratorium Animalis Scientia Sinica,2011,19(3):216-219,278. |
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| Authors: | YANG Ai-dong LI Wen-wen WANG Li-xia WANG Dong-ying WU Zhong-hua SU Zhong-hao Pang Hui-fang |
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| Institution: | YANG Ai-dong,LI Wen-wen,WANG Li-xia,WANG Dong-ying,WU Zhong-hua,SU Zhong-hao,Pang Hui-fang(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China) |
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| Abstract: | Objective To investigate the changes of TLR4 and CD14 mRNA expression in the rats with acute lung injury(ALI) induced by lipopolysaccharide(LPS).Methods Twenty-four male Wistar rats were randomly divided into two groups: normal control group and LPS model group.Each group had two subgroups,4 hours and 8 hours after LPS injection.The ALI model was established by intravenous injection of LPS(10 mg/kg) through the tail vein.The arterial blood gas graph(ABGG) and lung-body index were analyzed.The expressions of... |
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| Keywords: | CD14 |
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