| 重组降血压肽发酵培养基国产化的研究 |
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| 引用本文： | 付海涛,刘冬,彭光华,张声华.重组降血压肽发酵培养基国产化的研究[J].氨基酸和生物资源,2007,29(1):58-63,67. |
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| 作者姓名： | 付海涛 刘冬 彭光华 张声华 |
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| 作者单位： | 华中农业大学食品科技学院,武汉,430070;深圳职业技术学院应用化学与生物技术学院,深圳,518055 |
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| 基金项目： | 广东省社会发展公关项目及深圳市科技三项基金项目 |
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| 摘 要： | 利用国产培养基原料在摇瓶发酵中对重组大肠杆菌E.coliBL21生产降血压肽的发酵培养基进行了研究,根据试验结果得出优化的国产培养基配方质量分数为:酵母粉3%,蛋白胨2.5%,葡萄糖1%,NaCl0.5%。选择使用优化国产培养基虽然比进口培养基的表达量下降了15%,但是生产成本大大降低,为下一步的工业化生产打下了基础。
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| 关 键 词： | 降血压肽 培养基 发酵 |
| 文章编号： | 1006-8376（2007）01-0058-06 |
| 修稿时间： | 2006-12-05 |
Domestic Production Research of Fermentation Medium of Recombinant E. coli BL21 Expressing Antihypertensive Peptide |
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| FU Hai-tao,LIU Dong,PENG Guang-hua,ZHANG Sheng-hua.Domestic Production Research of Fermentation Medium of Recombinant E. coli BL21 Expressing Antihypertensive Peptide[J].Amino Acids & Biotic Resources,2007,29(1):58-63,67. |
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| Authors: | FU Hai-tao LIU Dong PENG Guang-hua ZHANG Sheng-hua |
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| Institution: | 1 College of Food Science and Technology, Huazhong Agriculture University, Wuhan 430070 ; 2 The School of Applied Chemistry and Biological Technology, Shenzhen Polytechnic, Shenzhen 518055 China |
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| Abstract: | The fermentation medium of the recombinant bacteria(E.coli BL21) using domestic ingredients was studied.By Orthogonal test,the optimal culture medium was: yeast extract 3%,tryptone 2.5%,glucose 1%,sodium chloride 0.5%.Although the expression of the domestic medium was 15% lower than the foreign one,the cost degraded greatly. |
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| Keywords: | antihypertensive peptide culture medium fermentation |
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