Somatic embryogenesis and plant regeneration of turf-type bermudagrass: Effect of 6-benzyladenine in callus induction medium |
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Authors: | Chaudhury Ashok Qu Rongda |
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Institution: | (1) Department of Crop Science, North Carolina State University, Raleigh, NC 27695-7620, USA;(2) Present address: Bioresources and Biotechnology Division, Tata Energy Research Institute, Darbari Seth Block, Habitat Place, Lodhi Road, New Delhi-, 110003, India |
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Abstract: | In order to optimize tissue culture conditions for bermudagrass, an important warm-season turfgrass species, tissue culture
responses of young inflorescences of a hybrid bermudagrass cultivar `Tifgreen' (Cynodon dactylon×Cynodon transvaalensis) and a common bermudagrass cultivar `Savannah' (Cynodon dactylon) were investigated. When cultured on Murashige and Skoog medium with 4.52 to 13.57 μM (1–3 mg l-1) 2,4-D, young inflorescence segments yielded non-embryogenic calli which were unorganized and had loosely associated, long
tubular cells on the surface. However, inclusion of 6-benzyladenine (BA) in callus induction medium at a level of 0.044 μM
(0.01 mg l-1) induced formation of a compact, nodular embryogenic structure on approximately 20% of the calli. Calli with such a compact
embryogenic structure were highly regenerable. When young inflorescences smaller than 0.75 cm were cultured, the embryogenic
structure yielded green plantlets with regeneration rates of 79.5% and 83.3%, respectively for the two cultivars. All 96 plants
regenerated from calli induced in the BA-containing medium were green and morphologically normal. The embryogenic nature of
the compact structure was confirmed by scanning electron microscopy.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | Cynodon dactylon Cynodon dactylon×Cynodon transvaalensis cytokinin MS medium plant tissue culture |
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