The bialaphos biosynthetic genes ofStreptomyces hygroscopicus: Molecular cloning and characterization of the gene cluster |
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| Authors: | Takeshi Murakami Hiroyuki Anzai Satoshi Imai Atsuyuki Satoh Kozo Nagaoka and Charles J Thompson |
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| Institution: | (1) Pharmaceutical Research Laboratories, Meiji Seika Kaisha, Ltd., Morooka-cho, Kohoku-ku, 222 Yokohama, Japan;(2) Pharmaceutical Development Laboratories, Meiji Seika Kaisha Ltd., Horikawa-cho, Saiwai-ku, 210 Kawasaki, Japan;(3) Biogen S.A., P.O. Box, CH-1211 Geneva 24, Switzerland |
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| Abstract: | Summary We have isolated and studied the organization ofStreptomyces hygroscopicus genes responsible for the biosynthesis of the antibiotic herbicide bialaphos. Bialaphos production genes were cloned from
genomic DNA using a plasmid vector (pIJ702). Three plasmids were isolated which restored productivity toS. hygroscopicus mutants blocked at different steps of the biosynthetic pathway. Subcloning experiments using other nonproducing mutants showed
that four additional bialaphos production genes were also contained on these plasmids. A gene conferring resistance to bialaphos,
which was independently cloned using the plasmid vector pIJ61, and an antibiotic-sensitive host (S. lividans), was also linked to the production genes. Cosmids were isolated which defined the location of these genes in a 16 kb cluster. |
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| Keywords: | Streptomyces Antibiotics Bialaphos Resistance gene Biosynthetic gene cluster |
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