Mitotic and polytene chromosomes analysis of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) |
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Authors: | Antigone Zacharopoulou Antonios A Augustinos Waheed A A Sayed Alan S Robinson Gerald Franz |
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Institution: | (1) Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Agency’s Laboratories, Seibersdorf, Austria;(2) Department of Biology, University of Patras, Patras, 26500, Greece;(3) Present address: Biological Application Department, Nuclear Research Centre, Atomic Energy Authority, Cairo, Egypt |
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Abstract: | The Oriental fruit fly, Batrocera dorsalis s.s. (Hendel) is one of the most destructive agricultural pests, belonging to a large group of difficult to distinguish morphologically
species, referred as the B. dorsalis complex. We report here a cytogenetic analysis of two laboratory strains of the species and provide a photographic polytene
chromosome map from larval salivary glands. The mitotic complement consists of six chromosome pairs including a heteromorphic
sex (XX/XY) chromosome pair. Analysis of the polytene complement has shown a total of five polytene chromosomes (10 polytene
arms) that correspond to the five autosomes. The most important landmarks of each polytene chromosome and characteristic asynapsis
at a specific chromosomal region are presented and discussed. Chromosomal homology between B. dorsalis and Ceratitis capitata has been determined by comparing chromosome banding patterns. The detection of chromosome inversions in both B. dorsalis strains is shown and discussed. Our results show that the polytene maps presented here are suitable for cytogenetic analysis
of this species and can be used for comparative studies among species of the Tephritidae family. They also provide a diagnostic
tool that could accelerate species identification within the B. dorsalis complex and could shed light on the ongoing speciation in this complex. Polytene chromosome maps can facilitate the development
of biological control methods and support the genome mapping project of the species that is currently in progress. |
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