Synthesis of RNA containing polyadenylic acid sequences in preimplantation rabbit embryos |
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Authors: | Gilbert Schultz Cole Manes William E. Hahn |
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Affiliation: | 1. Department of Anatomy, University of Colorado Medical Center Denver, Colorado 80220 USA;2. Department of Pediatrics, University of Colorado Medical Center Denver, Colorado 80220 USA |
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Abstract: | Messenger RNA synthesis has been estimated by assaying polyadenylic acid (poly A)-rich sequences in heterogeneous RNA from preimplantation rabbit embryos. Poly A containing RNAs are synthesized at least as early as the 16-cell stage and continue to be made through blastocyst formation and maturation. Sixty to 78% of the heterogeneous polysomal RNA in blastocysts contain poly A sequences. The portion of the heterogeneous RNA containing poly A sequences does not appear to change markedly between cleavage and blastocyst stages of development. Poly Arich sequences are greater than 4 S and consist of at least 84% adenine residues. RNA molecules ranging from 6 S to greater than 28 S contain poly A sequences. |
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Keywords: | To whom requests for reprints should be addressed. |
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