Optimization of medium composition for the production of exopolysaccharides from Phellinus baumii Pilát in submerged culture and the immuno-stimulating activity of exopolysaccharides |
| |
Authors: | Jianguang Luo Jun Liu Chunling Ke Deliang Qiao Hong Ye Yi Sun Xiaoxiong Zeng |
| |
Affiliation: | aCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China |
| |
Abstract: | Optimization of medium composition for the production of exopolysaccharides (EPS) from Phellinus baumii Pilát in submerged culture and the immuno-stimulating activity of EPS were carried out. Firstly, the medium components having significant effect on EPS production were screened out to be glucose, yeast extract and diammonium oxalate monohydrate by using a 2(7−3) fractional factorial design. Secondly, the concentrations of the three factors were optimized using central composite design in response surface methodology. As results, a quadratic model was found to fit for EPS production, and the optimal medium composition was determined as following (g/l): 34.12 glucose, 4 peptone, 5.01 yeast extract, 0.88 diammonium oxalate monohydrate, 0.75 MgSO4 and 1 KH2PO4 and 0.0075 thiamine (VB1). A yield of 2.363 ± 0.04 g/l for EPS was observed in verification experiment. Finally, EPS from P. baumii Pilát was found to have direct immuno-stimulating activity in vitro on splenocyte proliferative response and acid phosphatase activity in peritoneal macrophages in a dose-dependent manner. |
| |
Keywords: | Phellinus baumii Pilá t Exopolysaccharide Medium composition Statistical optimization design Immuno-stimulating activity |
本文献已被 ScienceDirect 等数据库收录! |
|