Synthesis of a mesoporous functional copolymer bead carrier and its properties for glucoamylase immobilization |
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Authors: | Yongxiao Bai Yanfeng Li Lin Lei |
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Institution: | (1) Institute of Material Science and Engineering, Lanzhou University, Lanzhou, 730000, China;(2) Institute of Biochemical Engineering & Environmental Technology, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China |
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Abstract: | A series of mesoporous and hydrophilic novel bead carriers containing epoxy groups were synthesized by modified inverse suspension
polymerization. Glycidyl methacrylate and acryloyloxyethyl trimethyl ammonium chloride were used as the monomers, and divinyl
benzene, allyl methacrylate, and ethylene glycol dimethacrylate as crosslinking agents, respectively. The resulting carriers
were employed in the immobilization of glucoamylase (Glu) with covalent bond between epoxy groups and enzymes. The activity
recovery of the three series of immobilized Glus could reach 76%, 79%, and 86%, respectively. The immobilized Glus exhibit
excellent stability and reusability than that of the free ones. |
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Keywords: | Mesoporous bead carrier Functional copolymer Inverse suspension polymerization Immobilized glucoamylase Enzymatic reaction |
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