Salt tolerance conferred by overexpression of Arabidopsis vacuolar Na+/H+ antiporter gene AtNHX1 in common buckwheat (Fagopyrum esculentum) |
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Authors: | Li-Hong Chen Bo Zhang Zi-Qin Xu |
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Affiliation: | (1) Shaanxi Provincial Key Laboratory of Biotechnology, Northwest University, Xi’an, 710069, P.R. China |
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Abstract: | Agriculture productivity is severely affected by soil salinity. One possible mechanism by which plants could survive salt stress is to compartmentalize sodium ions away from the cytosol. In the present work, transgenic buckwheat plants overexpressing AtNHX1, a vacuolar Na+/H+ antiporter gene from Arabidopsis thaliana, were regenerated after transformation with Agrobacterium tumefaciens. These plants were able to grow, flower and accumulate more rutin in the presence of 200 mmol/l sodium chloride. Moreover, the content of important nutrients in buckwheat was not affected by the high salinity of the soil. These results demonstrated the potential value of these transgenic plants for agriculture use in saline soil. |
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Keywords: | Fagopyrum esculentum Moench Salt tolerance Na+/H+ antiporter Rutin HPLC |
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