Separation of yeasts by addition of flocculent cells of Saccharomyces cerevisiae |
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Authors: | Annelies Mortier Eduardo V. Soares |
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Affiliation: | (1) Department of Biochemistry-Microbiology, KaHo St.-Lieven, Industrial Engineering, Gebroeders Desmetstraat 1, 9000 Ghent, Belgium;(2) Chemical Engineering Department, Superior Institute of Engineering from Porto Polytechnic Institute, Rua Dr António Bernardino de Almeida, 431, 4200-072 Porto, Portugal;(3) IBB-Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal |
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Abstract: | Separation of yeast cells using a co-flocculation process was investigated. Co-flocculation is a fast process (within few minutes), occurs in a broad pH range (3.0–8.0) and requires a small amount of calcium (0.1 mM). Agitation affects yeast aggregation; however, an agitation between 60 rev/min and 160 rev/min has only a little influence on the co-flocculation process. The ratio flocculent/non-flocculent cells that induced the settling of 50 and 90% of the cells of S. cerevisiae was 1:7 and 1:1, respectively. Separation of non-flocculent cells can be carried out at any time of the growth cycle. No difference in the efficiency of co-flocculation carried out in buffer (pH 4.0 with 10 mM calcium) or in 48 h-fermented broth was observed. Flocculent cells of Saccharomyces cerevisiae had the ability to sediment non-flocculent cells of S. cerevisiae and Kluyveromyces marxianus, which shows the suitability of the co-flocculation process for separation of different kinds of non-flocculent cells. |
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Keywords: | Adhesion Aggregation Cell separation Co-flocculation Sedimentation Yeast |
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