Measure ofLegionella pneumophila activity in situ |
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Authors: | Carl B. Fliermans Reginald J. Soracco Daniel H. Pope |
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Affiliation: | (1) E. I. du Pont de Nemours & Co., Savannah River Laboratory, 29808 Aiken, South Carolina, USA;(2) Rensselaer Polytechnic Institute, 12181 Troy, New York, USA |
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Abstract: | Detection ofLegionella pneumophila by serogroup-specific fluorescent antibodies was combined with a tetrazolium dye (INT) to measure electron transport activity. The biological uptake and reduction of the INT dye was studied in pure cultures and in natural water samples with respect to temperature. Uptake was complete within 60 min. Controls inhibited with formaldehyde demonstrated little activity. Both the in vitro and in situ determinations suggested that the electron transport system ofLegionella was active over a temperature range of 25 to 60°C. |
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