Somatic embryogenesis in Vicia faba L. |
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Authors: | Miroslav Griga Marie Kubaláková Eva Tejklová |
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Affiliation: | (1) Research and Breeding Institute of Technical Crops and Legumes, OSEVA-Plant Breeding and Seed Corporation, CS-787 01 Sumperk-Temenice;(2) Institute of Experimental Botany, Czechoslovak Academy of Sciences, Sokolovská 6, CS-772 00 Olomouc, Czechoslovakia |
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Abstract: | The paper describes a method of somatic embryo induction in callus and suspension cultures of Vicia faba L. Callus was induced from immature cotyledons (green maturity stage) of white-flowering horse bean lines cultured on L2 medium (Phillips and Collins 1979) supplemented with 1% sucrose, 0.7% agar and different concentrations of 2,4-dichlorophenoxyacetic acid. The medium with 2.5 M 2,4-Dichlorophenoxyacetic acid was found optimum for embryogenic callus induction. Somatic embryos developed after transfer of the callus to media lower or zero 2,4-Dichlorophenoxyacetic acid and increased level of sucrose (2.5%). The release of somatic embryos from the callus was more apparent after transfer to liquid medium. There were various stages of somatic embryo development, i.e. globular, heart-shaped and torpedo ones. |
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Keywords: | Vicia faba L. callus culture suspension culture somatic embryogenesis |
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