Genetic transformation of Arabidopsis lyrata: specific expression of the green fluorescent protein (GFP) in pistil tissues |
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Authors: | Isabelle Fobis-Loisy Pierre Chambrier Thierry Gaude |
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Institution: | (1) Laboratoire de Reproduction et Développement des Plantes, UMR 5667 CNRS-INRA-ENSL-UCB Lyon I, IFR 128 BioSciences Lyon-Gerland, ENS Lyon 46, Allée d’Italie, 69364 Lyon cedex 07, France |
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Abstract: | Arabidopsis thaliana has become widely used as a model system for plant biology. Recent phylogenetic studies led to a severe revision of the systematic
relationships across species of the Brassicaceae family. This provided an opportunity to examine close relatives of A. thaliana and to study the function and molecular evolution of genes that play roles in ecology and speciation. In this context, developing
tools to genetically transform “non-model plants” appears as a major issue to ascertain gene function. Here, we report a method
to transform A. lyrata, one of the closest relatives of A. thaliana. |
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Keywords: | Arabidopsis lyrata transformation GFP expression Stigma |
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