Determination of glycogen with the anthrone reagent |
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| Authors: | J H Roe R E Dailey |
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| Affiliation: | 1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;2. Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China;3. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;4. Fish Nutrition Research Laboratory, Department of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, Canada;5. Institute of Fisheries Science, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850002, China |
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| Abstract: | - 1.1. A method for the determination of glycogen has been developed. The procedure involves digestion of tissue in boiling NaOH, deproteinization of the alkaline digest with HClO4, precipitation with alcohol, washing with alcohol, and color production with anthrone reagent.
- 2.2. Experiments with glycogen added to liver, skeletal muscle, and heart showed mean recoveries of 92 to 101%.
- 3.3. The method combines the advantages of the classical alkaline extraction technique and the rapid colorimetric anthrone procedure. The possibility of interference from glycoprotein is removed by the perchloric acid treatment.
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