Differential effects of urea on yeast and bovine copper, zinc superoxide dismutases, in relation to the extent of analogy of primary structure. |
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Authors: | D Barra F Bossa F Marmocchi F Martini A Rigo G Rotilio |
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Institution: | 1. Istituto di Chimica Biologica, Università di Camerino Italy;2. Istituto di Chimica Biologica, Università di Roma Italy;3. Centro di Biologia Molecolare del C.N.R. Italy;4. Istituto di Chimica Fisica, Università di Venezia Italy |
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Abstract: | Incubation in 8M urea (pH 7.4) inactivated yeast Cu, Zn superoxide dismutase with biphasic first order kinetics (k for the decrease from 100% to 16% activity = 6.5 × 10?3 min?1; k for the decrease from 16% to 0.1% activity = 2.5 × 10?3 min?1). The inactivation was fully reversible on dilution with or dialysis against urea-free buffer. No inactivation was shown to occur in similar experiments with the bovine Cu, Zn enzyme. EPR spectra recorded immediately after addition of 8M urea showed a more axial line shape and a higher A″ of the copper signal typical of the native enzyme. In the case of the yeast enzyme, this change was more pronounced and further incubation led to a new type of copper signal, typical of the inactivated enzyme. All EPR changes were reversible. Comparative analysis of the amino acid sequence of the two enzymes showed substantial identity of the protein regions contributing the ligands to the metals and the disulfide bridge. Differential destabilization of active sites by urea should be due to replacements in other protein segments, such as the three C-terminal and some N-terminal residues. |
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