| 实时定量PCR技术及应用 |
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| 引用本文： | 杨凤秋,朱正歌. 实时定量PCR技术及应用[J]. 生物学杂志, 2006, 23(3): 44-46 |
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| 作者姓名： | 杨凤秋 朱正歌 |
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| 作者单位： | 河北师范大学生命科学学院,石家庄,050821 |
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| 基金项目： | 河北师范大学博士基金资助项目(L2001B01) |
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| 摘 要： | 实时定量PCR(Real-tim e Quantitative Polym erase Chain Reaction,RQ-PCR),是20世纪90年代中期发展起来的基于PCR技术的利用不同的荧光检测来给核酸定量的技术。克服了传统PCR的许多不足,能准确敏感地检测模板浓度,DNA拷贝数和检测基因变异。综述了RQ-PCR技术的原理,RQ-PCR实时定量检测系统及应用。
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| 关 键 词： | 实时定量 PCR 检测 应用 |
| 文章编号： | 1008-9632(2006)03-0044-03 |
| 收稿时间： | 2005-09-24 |
| 修稿时间： | 2005-09-24 |
Real-time quantitative PCR and its applications |
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| YANG Feng-qiu,ZHU Zheng-ge. Real-time quantitative PCR and its applications[J]. Journal of Biology, 2006, 23(3): 44-46 |
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| Authors: | YANG Feng-qiu ZHU Zheng-ge |
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| Affiliation: | College of Life Sciences, Hebei Normal University, Shijiazhuang 050821, China |
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| Abstract: | Developed in the mid 1990s for the analysis and quantification of nucleic acid,Real-time quantitative PCR is a molecular biological technique based on different fluorescence gaining rapidly in popularity.The technique can measure template concentration,DNA copy number,genome mutation and so on.RQ-PCR has reformed the conventional PCR and has many advantages such as rapidness sensitivity and securacy.In this paper the basic principles and the applications of this technique were introduced. |
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| Keywords: | real-time quantitative PCR monitor application |
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