Phospholipase A2-like catalytic antibody |
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| Authors: | Isomura S Ito K Haruna M |
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| Institution: | Faculty of Pharmacy, Meijo University, Yagotoyama, Tempaku, Nagoya, Japan. |
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| Abstract: | The phospholipase A2-like catalytic antibody 13C2-1F6 was elicited against the hapten 1 as the transition state analog for the hydrolysis of the C2 ester in the phospholipid. The Michaelis-Menten kinetics for the hydrolysis of the phospholipid 2 by 13C2-1F6 afforded a kcat of 1.0 x 10(-2) min(-1) and aKm of 71 microM. This antibody hydrolyzes the C2 ester in (R)-2, regio- and enantioselectively. |
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