Synergistic promotion of gibberellin and cytokinin on direct regeneration of floral buds from in vitro cultures of sepal segments in sinningia speciosa hiern |
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Authors: | Ji-Liang Pang Li-Lin Wang Jiang-Qin Hu Tai-He Xiang Hai-Man Liang |
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Institution: | (1) School of Life Sciences, Hangzhou Normal College, 310018 Hangzhou, People's Republic of China;(2) College of Life Sciences, Zhejiang University, 310012 Hangzhou, People's Republic of China |
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Abstract: | Summary This study reports the direct regeneration of flower buds from cultured sepal segments of Sinningia speciosa Hiern. Two types of floral bud regeneration were observed: regeneration of floral buds only (designed as BF) and regeneration of both floral and vegetative buds (designed as BF+V). The capacity of BF regeneration was closely related to the location of sepal segments and the concentration of exogenous gibberellin (GA3) and cytokinin in the medium. On the medium containing 1.0mgl−1 GA3, the addition of 6-benzyladenine (BA) significantly increased the frequency of total flower bud (BF+BF+V) formation, with the frequency up to 91.5% in the presence of 0.4mgl−1 BA. On the medium containing 0.1mgl−1 BA, the addition of GA3 also increased the frequency of total flower bud regeneration, with the frequency up to 74.3% in the presence of 1.0mgl−1 GA3, but no further increase in regeneration was observed when the GA3 concentration was higher than 1.0mgl−1. The capacity of BF regeneration from different locations of sepal segments was differential. The adaxial part of sepal segments gave rise to
the highest frequency of 56.7 and 84.3% for BF and BF+BF+V, respectively. |
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Keywords: | benzyladenine direct regeneration of floral buds gibberellin Sinningia speciosa Hiern |
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