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Environmental DNA (eDNA): Powerful technique for biodiversity conservation
Institution:1. Kerala University of Fisheries and Ocean Studies, Faculty of Fisheries, Panangad, Cochin, Kerala 682 506 India;2. Department of Biotechnology, Delhi Technological University, Delhi 110 042 India;3. College of Fisheries, Acharya Narendra Deva University of Agriculture and Technology, Ayodhya, Uttar Pradesh 224 229 India;4. ICAR-National Bureau of Fish Genetic Resources, Lucknow 226 002, Uttar Pradesh, India
Abstract:Environmental DNA metabarcoding is a non-invasive method for discovering and identifying rare and endangered species in a variety of ecosystems, including aquatic environments, based on the retrieval of genetic traces emitted into the environment by animals. Environmental (e) DNA research has grown in popularity over the last decade as a result of a rise in the number of studies that employ DNA taken from the environment, particularly in freshwater and marine ecosystems. In terms of detecting diversity patterns, we may claim that DNA retrieved from the environment (eDNA) is altering the game. For resource management in fisheries, information on species composition and biomass/abundance of commercially and noncommercially harvested species is critical. The eDNA is a truly non-invasive method that inflicts no damage on the species or habitats under study even during sampling, the eDNA technique never harms any ecosystems or threatened species. This novel molecular method never affects any endangered species or ecosystem during sampling. Environmental DNA analysis has become more widely accepted and is used in the detection of the presence and absence of aquatic macrofauna, such as freshwater and marine fish. This review study may aid researchers in better understanding the current state of eDNA technology. Despite the fact that various scientists have used eDNA to investigate the worldwide biodiversity of aquatic environments, no one in India is focusing on this new technology. We conclude that the eDNA technique has the potential to become a next-generation tool for biodiversity research and aquatic ecosystem conservation.
Keywords:Environmental DNA  Metabarcoding  Fish diversity  Biodiversity monitoring  Aquatic ecosystems
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