The Arabidopsis ortholog of the DEAH-box ATPase Prp16 influences auxin-mediated development |
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Authors: | Ryuji Tsugeki Shiho Terada |
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Institution: | Department of Botany; Graduate School of Science; Kyoto University; Sakyo-ku, Kyoto, Japan |
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Abstract: | In animals and yeasts, the DEAH-box RNA-dependent ATPase Prp16 facilitates pre-mRNA splicing. However, in Chlamydomonas reinhardtii and Caenorhabditis elegans, Prp16 orthologs are not important for general pre-mRNA splicing, but are required for gene silencing and sex determination, respectively. The CLUMSY VEIN (CUV) gene, which encodes a unique Prp16 ortholog in Arabidopsis thaliana, influences auxin-mediated development. A loss-of-function cuv-1 mutation tells us that CUV does not facilitate splicing of pre-mRNA substrates indiscriminately, but differentially effects splicing and expression of genes. Here we show that CUV influences root-meristem maintenance and planar polarity of root-hair positioning, both of which are processes regulated by auxin. We propose that Arabidopsis PRP16/CUV differentially facilitates the expression of genes, including genes involved in auxin biosynthesis, transport, perception and signaling, and that in this way it influences auxin-mediated development. |
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Keywords: | Arabidopsis auxin CLUMSY VEIN planar polarity Prp16 pre-mRNA splicing root hair |
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