Epigenetic silencing of olfactomedin-4 enhances gastric cancer cell invasion via activation of focal adhesion kinase signaling |
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Authors: | Li-Li Guo Zhao-Cai He Chang-Qing Yang Pei-Tang Qiao Guo-Ling Yin |
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Institution: | 1.Department of Gastroenterology, Heping Hospital, Changzhi Medical College;2.Department of General Surgery, Heping Hospital, Changzhi Medical College;3.Department of radiotherapy, Heping Hospital, Changzhi Medical College, Changzhi, Shanxi 046000, China |
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Abstract: | Downregulation of olfactomedin-4 (OLFM4) is associated with tumor progression, lymph node invasion and metastases. However, whether or not downregulation of OLFM4 is associated with epigenetic silencing remains unknown. In this study, we investigate the role of OLFM4 in gastric cancer cell invasion. We confirm the previous result that OLFM4 expression is increased in gastric cancer tissues and decreases with an increasing number of metastatic lymph nodes, which are associated with OLFM4 promoter hypermethylation. Overexpression of OLFM4 in gastric cancer cells had an inhibitory effect on cell invasion. Furthermore, we found that focal adhesion kinase (FAK) was negatively correlated with OLFM4 in regards to lymph node metastasis in gastric cancer tissues. Also, inhibition of FAK induced by OLFM4 knockdown resulted in a decrease in cell invasion. Thus, our study demonstrates that epigenetic silencing of OLFM4 enhances gastric cancer cell invasion via activation of FAK signaling. BMB Reports 2015; 48(11): 630-635] |
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Keywords: | Focal adhesion kinase Gastric cancer Invasion Matrix metalloproteinases Metastasis Olfactomedin-4 |
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