Ghrelin and obestatin can promote human ovarian granulosa cell functions and FSH effects |
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| Affiliation: | 1. Constantine The Philosopher University in Nitra, Tr. A. Hlinku 1, 949 74 Nitra, Slovakia;2. King Saud University, College of Science, Department of Zoology, Riyadh 11451, Saudi Arabia;1. Maimonides Institute for Biomedical Research of Cordoba (IMIBIC), Córdoba, Spain;2. Endocrinology and Nutrition Service, Reina Sofia University Hospital, Córdoba, Spain;3. Department of Cell Biology, Physiology, and Immunology, University of Córdoba, Córdoba, Spain;4. CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Córdoba, Spain;5. Reina Sofia University Hospital, Córdoba, Spain;6. Pathology Service, Reina Sofia University Hospital, Córdoba, Spain;7. Medical Oncology Service, Reina Sofia University Hospital, Córdoba, Spain;8. Thoracic Surgery and Lung Transplantation Unit, Reina Sofia University Hospital, Córdoba, Spain;9. Department of Internal Medicine, Division of Endocrinology, Erasmus MC, Rotterdam, The Netherlands;1. The State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China;2. Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73126, USA;3. College of Life Sciences, Nankai University, Tianjin, 300071, China;4. Stephenson Cancer Center, Oklahoma City, OK, 73104, USA;1. Chair for DNA Research, Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;1. Universidade Federal do Ceará (UFC), Departamento de Física, Campus do Pici, Fortaleza, CE, C.P. 6030, 60455-760, Brazil;2. Departamento de Física Teórica and IFIC, Centro Mixto Universitat de Valencia - CSIC, Universitat de Valencia, Burjassot-46100, Valencia, Spain;1. Biosanitary University Research Institute (INUBE), University of Extremadura, Badajoz, Spain;2. Service of Nephrology, Virgen del Puerto Hospital, Plasencia, Spain;3. Department of Nursing, University of Extremadura, Plasencia, Spain;4. Department of Nursing, University of Extremadura, Merida, Spain;5. Department of Anatomy, Cellular Biology and Zoology, University of Extremadura, Plasencia, Spain;6. Department of Medical and Surgical Therapeutics, University of Extremadura, Badajoz, Spain |
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| Abstract: | The aim of the present in-vitro experiments was to examine the direct influence of ghrelin and obestatin on viability, proliferation and progesterone release by human ovarian granulosa cells and their response to FSH administration. Human granulosa cells were cultured in presence of ghrelin or obestatin (both at 0, 1, 10 or 100 ng/ml) alone or in the presence of FSH (10 ng/ml). Cell viability, accumulation of proliferation markers PCNA and cyclin B1 and release of progesterone were analyzed by Trypan blue extrusion test, quantitative immunocytochemistry and ELISA. Ghrelin, obestatin and FSH up-regulated all the measured ovarian cell parameters. Moreover, both ghrelin and obestatin promoted all the stimulatory effects of FSH. The obtained results demonstrate the direct stimulatory action of ghrelin, obestatin and FSH on basic ovarian cell functions, as well as the ability of metabolic hormones to improve FSH action on human ovarian cells. |
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| Keywords: | Ghrelin Obestatin FSH Ovary Viability Proliferation Progesterone |
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