Thermochemical characteristics of 2,4-dichlorophenol degrading strain Pseudomonas GT241-1 of growth metabolism by microcalorimetry |
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Authors: | Yao Jun Liu Yi Zhong Wenhui He Jing Zhou Qin Qin Xia Wang Jianping Qu Songsheng Yu Ziniu |
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Institution: | Key Laboratory of Agricultural Microbiology of Chinese Agriculture Ministry, Huazhong Agricultural University, Wuhan 430070, P. R. China. yaojun64@163.com |
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Abstract: | By using LKB-2277 Bioactivity Monitor, ampoule method, the heat output of the growth metabolism of a 2.4-dichlorophenol degrading bacteria strain, Pseudomonas strain GT241-1, has been determined at 30 degrees C. From the thermogenic curves, it can be established that thermokinetic equation of their growth metabolism is Pt = Pt = 0 exp(k(m) t), dP/dt = k(m)P1, with the order of growth metabolism n = 1. The experimental results indicate that the relationship between the metabolic power (P) and the cell concentration (C), and relationship between the metabolic power of each cell (P0) and the cell concentration can be characterized by the following thermal equation: C = a + kP, InC = a' + k'P0 or dC/dP0 = KC1. The order of the P0-C equation n is also 1. These results are very significant in environmental sciences, biology and thermochemistry. |
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