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Phylogenetic identification of Sparganum proliferum as a pseudophyllidean cestode |
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| Authors: | Akatsuki Kokaze Hiroko Miyadera Kiyoshi Kita Rikuo Machinami Oscar Noya Belkisyolé Alarcón de Noya Munehiro Okamoto Toshihiro Horii Somei Kojima |
| Institution: | aDepartment of Parasitology, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, 108 Japan bDepartment of Pathology, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, Japan cSeccion de Biohelmintiasis, Instituto de Medicina, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela dThe Institute of Experimental Animal Sciences, Osaka University Medical School, Suita, Osaka, Japan eDepartment of Molecular Protozoology, Research Institute of Microbial Diseases, Osaka University Medical School, Suita, Osaka, Japan |
| Abstract: | Sparganum proliferum is characterized by continuous branching and budding, the resulting progeny invading all tissues of the human body, causing fatal sparganosis. Its life cycle, definitive hosts and the route of infection to humans have not yet been disclosed. Because its morphology is similar to Spirometra erinacei, the phylogeny of S. proliferum has been thought to be identical to or closely related to S. erinacei. However, the taxonomy of S. proliferum has not been established up to present due to the lack of definitive observations. In order to clarify the phylogenetic relationship between S. proliferum and S. erinacei, nucleotide sequences of mitochondrial NADH dehydrogenase subunit 3 gene (ND3) and four mitochondrial tRNA coding genes of S. proliferum and other pseudophyllidean cestodes were analyzed. The sequences of S. proliferum showed high similarity to those of S. erinacei, although they were clearly different from each other, indicating that the phylogeny of S. proliferum and S. erinacei is distinct. This is the first report showing the phylogenetic relationship among S. proliferum and other pseudophyllidean cestodes at the DNA sequence level. |
| Keywords: | Sparganum proliferum Spirometra erinacei Pseudophyllidea NADH dehydrogenase subunit 3 (ND3) gene Mitochondrial DNA Abbrevations: mtDNA mitochondrial DNA ND3 NADH dehydrogenase subunit 3 gene PCR polymerase chain reaction |
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