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Molecular cloning of the promoter region of the glyceraldehyde-3-phosphate dehydrogenase gene that contributes to the construction of a new transformation system in <Emphasis Type="Italic">Coriolus versicolor</Emphasis> |
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| Authors: | |
| Institution: | (1) Koshida Corporation, 2-2-9 Higashi-shinbashi, Minato-ku, Tokyo, 105-8642, Japan;(2) Forestry and Forest Products Research Institute, Tsukuba, Japan;(3) National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan;(4) Graduate School and School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan;(5) Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan |
| Abstract: | The white-rot basidiomycete Coriolus versicolor secretes several enzymes that participate in the degradation of lignin and various persistent organic pollutants. In this study, we attempted to establish a genetic transformation system with a homogenous promoter sequence for driving the gene for antibiotic resistance. We succeeded in cloning the promoter sequence of the gene for glyceraldehyde-3-phosphate dehydrogenase (gpd), which is expressed at high levels in C. versicolor. The expression vector pT7GPTHPT was constructed, which included a gene for resistance to hygromycin B under control of the gpd promoter. The successful selection of transformants on medium that contained hygromycin B indicated that the system should be useful not only for the genetic transformation of C. versicolor, but also for the overproduction of useful fungal enzymes such as laccase and peroxidase. |
| Keywords: | Coriolus versicolor Glyceraldehyde-3-phosphate dehydrogenase Homologous transformation Hygromycin B Promoter |
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