An improved detection system applied to the study of serum lipoproteins after single-step density gradient ultracentrifugation |
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Authors: | Janet Nilsson Valsa Mannickarottu Celina Edelstein Angelo M. Scanu |
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Affiliation: | 1. Department of Medicine, The University of Chicago, Pritzker School of Medicine, Chicago, Illinois 60637 USA;2. Department of Biochemistry, The University of Chicago, Pritzker School of Medicine, Chicago, Illinois 60637 USA |
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Abstract: | Starting from a single-spin ultracentrifugation procedure described previously (Foreman et al., J. Lipid Res. 18, 759, 1977), we have improved the system for detection of the fractions eluted from the gradient by monitoring them continuously at 280 nm. A graphic display readily permits assessment of the distribution of the lipoproteins and their quantification with the aid of a computer program. By the use of appropriate factors, one can convert absorbance readings into actual lipoprotein values which correspond well (±7% for low-density lipoproteins and ±5% for high-density lipoproteins) to those obtained by means of chemical analyses. The examples provided indicate the versatility of the method and its sensitivity (down to 0.1 ml of serum). |
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Keywords: | To whom correspondence should be addressed. |
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