红火蚁及其他两种蚂蚁毒腺细菌鉴定与多样性分析(英文)

【目的】本研究旨在分析红火蚁Solenopsis invicta毒腺细菌群落多样性，并与热带火蚁Solenopsis geminata和聚纹双刺猛蚁Diacamma rugosum比较毒腺细菌群落差异。【方法】采用Illumine Hiseq 2500测序平台对红火蚁(工蚁、有翅蚁和蚁后)、热带火蚁(工蚁)及聚纹双刺猛蚁(工蚁)毒腺细菌群落16S rRNA基因V3-V4区测序，基于测序数据进行生物信息学分析。【结果】变形菌门(Proteobacteria)在红火蚁工蚁、有翅蚁和蚁后以及热带火蚁工蚁毒腺中占优势，而厚壁菌门(Firmicutes)在聚纹双刺猛蚁工蚁毒腺中占优势。与红火蚁工蚁和有翅蚁相比，柔壁菌门(Tenericutes)在红火蚁蚁后毒腺中更丰富。广州红火蚁蚁后毒腺中假单胞杆菌Pseudomonas相对丰度显著高于其在有翅蚁及工蚁中的。螺原体Spiroplasma相对丰度在热带火蚁毒腺中显著高于在聚纹双刺猛蚁工蚁毒腺中的。毒腺中细菌多样性分析发现，芽孢杆菌属Bacillus和乳酸杆菌属Lactobacillus在广西红火蚁工蚁中的相对丰度显著高于在广州红火蚁工蚁中的。然而，乳酸杆菌属细菌在聚纹双刺猛蚁工蚁毒腺中的相对丰度显著高于广西的热带火蚁工蚁毒腺中的。【结论】毒腺细菌组成和多样性在3种不同种类蚂蚁工蚁和红火蚁不同品级中存在差异。

Identification and diversity analysis of bacteria in the venomglands of the fire ant,Solenopsis invicta,and two otherants (Hymenoptera: Formicidae)(In English)

【Aim】 The objective of this study is to assess the diversity of bacteria in the venom glands of the red imported fire ant, Solenopsis invicta, and to compare the bacterial communities in the venom glands with those of other two stinging ants, Solenopsis geminata and Diacamma rugosum. 【Methods】 16S rRNA V3-V4 regions of bacterial community of venom glands in S. invicta workers, alates and queens, S. geminata workers and D.ugosum workers were sequenced by using the Illumine Hiseq 2500 platform. Then, bioinformatic analysis was performed based on sequencing data. 【Results】 Proteobacteria were dominant in the venom glands of S. invicta workers, alates, and queens and S. geminata workers, while Firmicutes were abundant in the venom glands of D. rugosum workers. Tenericutes were more abundant in the venom glands of S. invicta queens than in the venom glands of workers and alates. The relative abundance of Pseudomonas in S. invicta queens was significantly higher than that in S. invicta alates and workers from Guangzhou. The relative abundance of Spiroplasma in the venom glands of S. geminata workers was significantly higher than that in D. rugosum workers. Microbial diversity analysis of venom glands of worker ants of Solenopsis species showed that the relative abundance of Bacillus and Lactococcus in S. invicta workers collected from Guangxi was significantly higher than that in S. invicta workers collected from Guangzhou. However, the relative abundance of Lactococcus in D. rugosum workers was significantly higher than that in workers of S. geminata from Guangxi. 【Conclusion】 The bacterial composition and diversity are different among the workers of three ant species, and among the three castes in red imported fire ant.