Organization of the mouse microfibril-associated glycoprotein-2 (MAGP-2) gene |
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| Authors: | Cheryl Frankfater Erika Maus Krisztina Gaal Fernando Segade Neal G Copeland Debra J Gilbert Nancy A Jenkins J Michael Shipley |
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| Institution: | (1) Division of Pulmonary and Critical Care Medicine, Department of Medicine, Barnes-Jewish Hospital at Washington University School of Medicine, 216 S. Kingshighway Blvd., St. Louis, Missouri 63110, USA, US;(2) Department of Cell Biology, Washington University School of Medicine, St. Louis, Missouri 63110, USA, US;(3) Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick, Maryland 21702, USA, US |
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| Abstract: | A 1.4-kb EST clone encoding mouse microfibril-associated glycoprotein-2 (MAGP-2), identified by its similarity with the reported
human cDNA, was used to screen a mouse 129 genomic bacterial artificial chromosome (BAC) library. The mouse gene contains
10 exons spanning 16 kb, located on the distal region of Chromosome (Chr) 6. The exons range in size from 24 to 963 bp, with
the ATG located in exon 2. The tenth and largest exon contains 817 bp of 3′ untranslated sequence, including a B2 repetitive
element. Northern analysis demonstrates abundant expression of MAGP-2 mRNA in skeletal muscle, lung, and heart. Sequence analysis
of additional cDNA clones suggests that the two mRNA forms of MAGP-2 in the mouse arise from alternative polyadenylation site
usage. The promoter does not contain an obvious TATA box, and the sequence surrounding the start site does not conform to
the consensus for an initiator promoter element. Additionally, the mouse promoter contains 22 copies of a CT dinucleotide
repeat sequence located ∼155 bp 5′ to exon 1.
Received: 27 August 1999 / Accepted: 2 November 1999 |
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