Isolation of free minus strands from Qβ-infected Escherichia coli |
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Authors: | TS Eikhom |
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Institution: | Department of Biochemistry, University of Bergen Årstadveien 19, 5000 Bergen, Norway |
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Abstract: | Free minus strands (minus strands not involved in a firm duplex structure) are produced in Escherichia coli infected with the RNA phage Qβ. These minus strands can be extracted from the cells under conditions of mild lysis and low salt concentrations, and can be purified by electrophoresis on polyacrylamide gels.The free minus strands are fully competent as template for the Qβ-replicase in the absence of host factors, directing the synthesis of plus strands. |
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