Rapid acquisition of Raman spectral maps through minimal sampling: applications in tissue imaging |
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| Authors: | Christopher J Rowlands Sandeep Varma William Perkins Iain Leach Hywel Williams Ioan Notingher |
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| Institution: | 1. School of Physics and Astronomy, University of Nottingham, University Park, NG7 2RD, Nottingham, U.K.;2. Dermatology Department, Nottingham University Hospital NHS Trust, QMC Campus, Derby Road, NG7 2UH, Nottingham, U.K.;3. Histopathology Department, Nottingham University Hospital NHS Trust, QMC Campus, Derby Road, NG7 2UH, Nottingham, U.K.;4. Centre of Evidence‐Based Dermatology, C Floor South Block, Nottingham University Hospital NHS Trust, QMC Campus, Derby Road, NG7 2UH, Nottingham, U.K. |
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| Abstract: | A method is presented for acquiring high‐spatial‐resolution spectral maps, in particular for Raman micro‐spectroscopy (RMS), by selectively sampling the spatial features of interest and interpolating the results. This method achieves up to 30 times reduction in the sampling time compared to raster‐scanning, the resulting images have excellent correlation with conventional histopathological staining, and are achieved with sufficient spectral signal‐to‐noise ratio to identify individual tissue structures. The benefits of this selective sampling method are not limited to tissue imaging however; it is expected that the method may be applied to other techniques which employ point‐by‐point mapping of large substrates. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
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| Keywords: | Raman spectroscopy image reconstruction computer assisted diagnosis microscopy |
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