The effect of the microsporidan, Nosema algerae, on Anopheles stephensi

A bioassay method was established to examine infectivity differences between different batches of Nosema algerae spores. The IC₅₀ of N. algerae spores produced in one unusual host, Heliothis zea, was the same as for spores from the normal mosquito host, Anopheles stephensi. Soil and sand bottoms caused an approximate 200–400 fold increase in the IC₅₀. Nosematosis had little effect on the survival of larvae and pupae but the adult life span was reduced to the extent that malaria transmission would be doubtful.