Simultaneous multi‐site two‐photon photostimulation in three dimensions |
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| Authors: | Mary Ann Go Christian Stricker Stephen Redman Hans‐A. Bachor Vincent Ricardo Daria |
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| Affiliation: | 1. John Curtin School of Medical Research, The Australian National University, Canberra, ACT 0200, Australia;2. Research School of Physics and Engineering, The Australian National University, Canberra, ACT 0200, Australia |
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| Abstract: | We demonstrate simultaneous multi‐site two‐photon photolysis of caged neurotransmitters with close to diffraction‐limited resolution in all three dimensions (3D). We use holographic projection of multiple focal spots, which allows full control over the 3D positions of uncaging sites with a high degree of localized excitation. Our system incorporates a two‐photon imaging setup to visualize the 3D morphology of the neurons in order to accurately determine the photostimulation sites. We show its application to studies of synaptic integration by performing simultaneous and controlled glutamate delivery at multiple locations on dendritic trees. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
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| Keywords: | Two‐photon photolysis holographic projection two‐photon imaging synaptic integration |
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