Effect of 50 S subunit proteins L5, L18 and L25 on the fluorescence of 5 S RNA-bound ethidium bromide |
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| Authors: | J. Feunteun R. Monier R. Garbett M. Le Bret J.B. Le Pecq |
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| Affiliation: | Institut de Recherches Scientifiques sur le Cancer B.P. n∘ 8 94800-Villejuif, France;Max-Planck-Institut für Molekulare Genetik 1 Berlin 33, Dahlem, Germany;Unité de Biochimie Institut Gustave-Roussy 94800-Villejuif, France |
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| Abstract: | Among the three Escherichia coli 50 S subunit proteins L5, L18 and L25, which have an affinity for 5 S RNA, only protein L18 exerts a strong effect on the fluorescence of 5 S RNA-ethidium bromide complexes, without changing the quantum yield of the fluorescence. Proteins L5 and L25, although they have little effect on the fluorescence, have a strong stabilizing influence on the 5 S RNA-L18 complex. The results are discussed in terms of the secondary and tertiary structures of 5 S RNA in relation to ribosomal protein binding. |
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