On the origin of the 1602 cm–1 Raman band of yeasts; contribution of ergosterol |
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| Authors: | Liang‐da Chiu Françoise Hullin‐Matsuda Toshihide Kobayashi Hajime Torii Hiro‐o Hamaguchi |
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| Institution: | 1. Department of Chemistry, School of Science, the University of Tokyo, 7‐3‐1, Hongo, Bunkyo‐ku, Tokyo 113‐0033 Japan;2. Lipid Biology Laboratory, RIKEN, 2‐1, Hirosawa, Wako, Saitama 351‐0198 Japan;3. UMR 870, INSERM, Lyon, France;4. Department of Chemistry, School of Education, Shizuoka University, 836 Ohya, Shizuoka, 422‐8529, Japan;5. Institute of Molecular Science and Department of Applied Chemistry, National Chiao Tung University, 1001, University Road, Hsinchu, Taiwan 300, R.O.C. |
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| Abstract: | The 1602 cm–1 Raman signature, which we call the “Raman spectroscopic signature of life” in yeasts, is a marker Raman band for cell metabolic activity. Despite the established fact that its intensity sensitively reflects the metabolic status of the cell, its molecular origin remained unclear. In this work, we propose ergosterol as the major contributor of the 1602 cm–1 Raman signature. The theoretical isotope shift calculation for ergosterol agreed with previous observations. Furthermore, experiments showed that the Raman spectrum of ergosterol corresponds very well with the depleting spectral component in yeast that behaves together with the 1602 cm–1 signature when the cells are under stress. This work implies that the 1602 cm–1 Raman signature could serve as an intrinsic ergosterol marker in yeasts for the study of sterol metabolism in vivo and in a label‐free manner, which could not be done by any other techniques at the current stage. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
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| Keywords: | Raman spectroscopy steroid Raman spectroscopic signature of life yeast |
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