Polymorphisms for human chromosomes 1 and Y: Feulgen and UV DNA measurements |
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| Authors: | JPM Geraedts PL Pearson M Van der Ploeg AM Vossepoel |
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| Institution: | 1. Instituut vor Anthropogenetica, University of Leiden, Leiden, The Netherlands;2. Department of Histochemistry and Cytochemistry, University of Leiden, Leiden, The Netherlands;3. Department of Biomedical Information Processing, University of Leiden, Leiden, The Netherlands |
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| Abstract: | Some individuals show considerable length differences between the homologues of chromosome no. 1 and length variations for the Y chromosome have also been found. The variabilities in length appear to be localized in the heterochromatic regions. The aim of this study was to distinguish between two phenomena postulated to contribute to length variations: (1) a genetically determined uncoiling of a chromosomal region, and (2) an increase in the chromosomal DNA content. By cytophotometry of photographic negatives the integrated absorbance of polymorphic and normal no. 1 and Y-chromosomes was compared, using chromosome no. 2 as standard. Microphotometry was carried out on both unstained chromosomes at 265 nm and on Feulgen-stained chromosomes at 546 nm. Both methods showed that the length polymorphisms studied are, in general, characterized by an increase in the chromosomal DNA. |
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