Heterodyne detected nonlinear optical imaging in a lock‐in free manner |
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| Authors: | Mikhail N Slipchenko Robert A Oglesbee Delong Zhang Wei Wu Ji‐Xin Cheng |
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| Institution: | 1. Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907 USA;2. Department of Chemistry, Purdue University, West Lafayette, IN 47907 USA |
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| Abstract: | We report a compact, cost‐effective tuned amplifier for frequency‐selective amplification of the modulated signal in heterodyne detected nonlinear optical microscopy. Our method improved the signal to noise ratio by an order of magnitude compared to conventional lock‐in detection, as demonstrated through stimulated Raman scattering imaging of live cells and tissues at the speed of 2 μsec/pixel. Application of the tuned amplifier to transient absorption microscopy is also demonstrated. The increased signal to noise ratio allowed epi‐detected in vivo imaging of myelin and blood in rat spinal cord with high spatial resolution. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
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| Keywords: | tuned amplifier coherent Raman scattering stimulated Raman scattering nonlinear microscopy heterodyne detection |
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