Effect of phenylhydrazine on the multiple hemoglobins of Rana catesbeiana tadpoles

Tadpoles of Rana catesbeiana immersed in or injected with phenylhydrazine show a preferential loss from the circulation of the electrophoretically fastest hemoglobin (Hb) and retention of the electrophoretically slower Hb's on polyacrylamide disk-gels.The electrophoretically slowest Hb has previously been found to be synthesized in the kidney while the other Hb's are synthesized in the liver. We postulate that the differential response of the tadpole hemoglobins to the action of phenylhydrazine results from the different Hb's being found in different red blood cell lines that originate in different erythropoietic tissues.