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Spectroscopic and histological evaluation of wound healing progression following Low Level Laser Therapy (LLLT) |
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| Authors: | Vijendra Prabhu Satish B S Rao Subhash Chandra Pramod Kumar Lakshmi Rao Vasudeva Guddattu Kapaettu Satyamoorthy Krishna K Mahato |
| Institution: | 1. Biophysics Unit, Manipal Life Sciences Centre, Manipal University, Manipal 576104, Karnataka, India;2. Division of Radiobiology & Toxicology, Manipal Life Sciences Centre, Manipal University, Manipal 576104, India;3. Department of Plastic Surgery & Burns, Kasturba Medical College, Manipal University, Manipal 576104, India;4. Department of Pathology, Kasturba Medical College, Manipal University, Manipal 576104, India;5. Department of Statistics, Manipal University, Manipal 576104, India;6. Department of Biotechnology, Manipal Life Sciences Center, Manipal University, Manipal 576104, India |
| Abstract: | The present study focuses on the evaluation of the effect of He‐Ne laser on tissue regeneration by monitoring collagen synthesis in wound granulation tissues in Swiss albino mice using analysis of laser induced fluorescence (LIF) and light microscopy techniques. The spectral analyses of the wound granulation tissues have indicated a dose dependent increase in collagen levels during the post‐wounding days. The histological examinations on the other hand have also shown a significant increase in collagen deposition along with the reduced edema, leukocytes, increased granulation tissue, and fibroblast number in the optimal laser dose treated group compared to the non‐illuminated controls. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
| Keywords: | Spectroscopic and microscopic technique fluorescence in biophysics therapeutic application collagen biomolecules |