Centriole movements in mammalian epithelial cells during cytokinesis |
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Authors: | Asta Björk Jonsdottir Roeland W Dirks Johannes Vrolijk Helga M Ögmundsdottir Hans J Tanke Jorunn E Eyfjörd Karoly Szuhai |
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Institution: | (1) Cancer Research Laboratory, Faculty of Medicine, University of Iceland, Vatnsmyrarvegi 16, 101 Reykjavik, Iceland;(2) Department of Molecular Cell Biology, Leiden University Medical Center, Einthovenweg 20, 2300 RC Leiden, the Netherlands |
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Abstract: | Background In cytokinesis, when the cleavage furrow has been formed, the two centrioles in each daughter cell separate. It has been suggested
that the centrioles facilitate and regulate cytokinesis to some extent. It has been postulated that termination of cytokinesis
(abscission) depends on the migration of a centriole to the intercellular bridge and then back to the cell center. To investigate
the involvement of centrioles in cytokinesis, we monitored the movements of centrioles in three mammalian epithelial cell
lines, HeLa, MCF 10A, and the p53-deficient mouse mammary tumor cell line KP-7.7, by time-lapse imaging. Centrin1-EGFP and
α-Tubulin-mCherry were co-expressed in the cells to visualize respectively the centrioles and microtubules. |
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