The secretory response through electric stimulation of differentiated PC12 rat pheochromocytoma cells transfected with neuropeptide Y fused with enhanced green fluorescent protein |
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| Authors: | Mizuno Atsushi Mie Masayasu Yanagida Yasuko Aizawa Masuo Kobatake Eiry |
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| Affiliation: | (1) Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan |
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| Abstract: | Exocytosis in pheochromocytoma cells was induced by electric stimulation. To chase the movement of vesicles by electric stimulation, dense-core secretory vesicles were visualized by expression of the fusion protein between neuropeptide Y and enhanced green fluorescent protein (EGFP) in these differentiated PC12 rat pheochromocytoma cells. When the cells were stimulated with constant voltage potential at –300 mV, the movement of dense-core secretory vesicles could be regulated. |
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| Keywords: | dense-core secretory granules electric stimulation enhanced green fluoresent protein exocytosis pheochromocytoma cell |
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