Binding of Chlorpromazine to S-100 Protein |
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Authors: | Rosario Donato |
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Institution: | Institute of Anatomy, Medical School, Perugia, Italy |
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Abstract: | Chlorpromazine (CPZ) induces in S-100 conformational changes resulting in the exposure of titratable SH groups of the protein to the solvent. This effect is even greater in the presence of Mg2+ +/- Ca2+. S-100 possesses binding sites for CPZ. The binding of CPZ to 3 microM S-100 is half-saturated by 0.18 microM CPZ in the presence of Mg2+ plus Ca2+ and by 0.24 microM CPZ in the presence of Mg2+ plus EGTA. The extent of the binding is greater in the presence of Ca2+ than in the presence of EGTA, especially at low CPZ concentrations. |
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Keywords: | S-100 protein Phenothiazines Conformational changes Binding |
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