Gene-specific silencing induced by parallel complementary RNA in <Emphasis Type="Italic">Pseudomonas aeruginosa</Emphasis> |
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Authors: | Yi Shuang Liu Yue Qin Zhang Li Xia Yang Tian Jue Yao Chun Ling Xiao |
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Institution: | (1) Institute of Medicinal Biotechnology, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, 100050, China; |
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Abstract: | To investigate whether parallel complementary RNA (pRNA) could induce gene-specific silencing in Pseudomonas aeruginosa, pRNA of the mexA gene was expressed in it. Compared to the control strains, the strain expressing pRNA of mexA showed a 50% decrease in minimum inhibitory concentrations (MICs) of several antimicrobial agents and a twofold increase
in the initial accumulation rate of ethidium bromide, all of which are substrates of the MexAB-OprM efflux pump. These results
suggest that gene-specific silencing was induced by pRNA. This is the first time that such a route for gene silencing has
been reported in a bacterium other than Escherichia coli. Gene-specific silencing induced by pRNA may be useful as a novel biotechnology tool for gene regulation in prokaryotes. |
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