Isolation and characterization of microsatellite loci from a potential biofuel plant Miscanthus sinensis (Poaceae) |
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Authors: | Kuo-Hsiang Hung Tzen-Yuh Chiang Chi-Te Chiu Tsai-Wen Hsu Chuan-Wen Ho |
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Institution: | (1) Department of Life Sciences, Cheng-Kung University, 1, University Road, Tainan, 701, Taiwan;(2) Taiwan Endemic Species Research Institute, Nantou, 552, Taiwan; |
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Abstract: | Miscanthus sinensis (Poaceae) is typical of many dominant grasses of East Asia. Due to its characteristics of fast growth and high biomass, Miscanthus, a C4 plant, has been long explored for the potential usage as biofuel. In this study, we described the development of nine
microsatellite loci from M. sinensis for genetic studies. These new markers were tested in 25 individuals of five populations in three varieties. The number of
alleles ranged from 3 to 8. The expected (H
E) and observed (H
O) heterozygosities were 0.37–0.78 and 0.05–0.52, respectively. All microsatellite loci are significantly deviated from Hardy–Weinberg
expectations likely due to the population structure within samples. Linkage disequilibrium between four loci pairs were observed
from the total 36 pairwise comparisons of loci. Cross-species transferability revealed that all loci can be applied to its
closely related species, M. floridulus. |
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Keywords: | |
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