Assessing the suitability of antibiotic resistance markers and the indirect ELISA technique for studying the competitive ability of selected Cyclopia Vent. rhizobia under glasshouse and field conditions in South Africa |
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Authors: | Amy C Spriggs and Felix D Dakora |
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Institution: | (1) Botany Department, University of Cape Town, Private Bag, Rondebosch 7701, Cape Town, South Africa;(2) Chemistry Department, Tshwane University of Technology, Private Bag X680, Pretoria, 0001, South Africa |
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Abstract: | Background Symbiotic N2 fixation in legumes is constrained by many factors, including the paucity of suitable soil rhizobia To maximise
growth of legume species therefore often requires the application of effective rhizobia as inoculants. But where native strains
out-compete introduced rhizobia for nodule formation, it is important that the competitiveness of selected strains is tested
in the field and glasshouse prior to their recommendation as commercial inoculants. However the methodology for strain identification
inside nodules has often proved difficult and thus limited this field of research. In this study, the suitability of the antibiotic
resistance technique (both intrinsic low-resistance fingerprinting and high-resistance marking) and the serological indirect
ELISA method were assessed for their ability to detect selected Cyclopia rhizobia under glasshouse and field conditions. The
four rhizobial strains that were used, namely PPRICI3, UCT40a, UCT44b and UCT61a, were isolated from wild Cyclopia species
growing in the Western Cape fynbos of South Africa. |
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