Nonuniform size distribution of nascent globin peptides, evidence for pause localization sites, and a cotranslational protein-folding model |
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Authors: | Igor A Krasheninnikov Anton A Komar and Ivan A Adzhubei |
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Institution: | (1) Department of Molecular Biology, Faculty of Biology, Moscow State University, 119899 Moscow, USSR |
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Abstract: | Examination of nascent globin peptides accumulatingin vitro during globin synthesis in rabbit reticulocyte lysates was carried out. A view was supported that nonrandom distribution of codons with different usage frequencies in mRNA may determine the messenger's translation kinetics. Regions of reduced translation of - and -globin polypeptide chains were localized, and the cotranslational protein-folding model suggested previously was substantiated. An active conjunction of synthesis and folding of proteins was proposed as one of the main destinations of a translation nonuniformity. |
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Keywords: | Nonrandom codon utilization nonuniform translation nascent peptides three-dimensional protein structure cotranslational folding |
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