Detection of linkage between marker loci and loci affecting quantitative traits in crosses between segregating populations |
| |
Authors: | J. S. Beckmann M. Soller |
| |
Affiliation: | (1) Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, 50250 Bet Dagan, Israel;(2) Department of Genetics, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel |
| |
Abstract: | Summary By making use of pedigree information and information on marker-genotypes of the parent and F-1 individuals crossed to form an F-2 population, it is possible to carry out a linkage analysis between marker loci and loci affecting quantitative traits in a cross between segregating parent populations that are at fixation for alternative alleles at the QTL, but share the same alleles at the marker loci. For two-allele systems, depending on marker allele frequencies in the parent populations, 2–4 times as many F-2 offspring will have to be raised and scored for markers and quantitative traits in order to provide power equivalent to that obtained in a cross between fully inbred lines. Major savings in number of F-2 offspring raised can be achieved by scoring each parent pair for a large number of markers in each chromosomal region and scoring F-1 and F-2 offspring only for those markers for which the parents were homozygous for alternative alleles. For multiple allele systems, particularly when dealing with hypervariable loci, only 10%–20% additional F-2 offspring will have to be raised and scored to provide power equivalent to that obtained in a cross between inbred lines. When a resource population contains novel favorable alleles at quantitative trait loci that are not present (or rare) in a commercial population, analyses of this sort will enable the loci of interest to be identified, mapped and manipulated effectively in breeding programs.Contribution no. 2124-E, 1987 series from The Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel |
| |
Keywords: | Restriction fragment length polymorphisms (RFLPs) Variable number tandem repeat regions (VNTRs) Quantitative trait loci (QTL) Marker-QTL linkage |
本文献已被 SpringerLink 等数据库收录! |
|