Effect of strand orientation on the interaction of berenil with DNA triple helices |
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Authors: | Maurice Durand Edouard Seche Jean Maurizot |
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Affiliation: | Centre de Biophysique Moléculaire, Laboratoire conventionné avec l'Université d'Orléans et affilié à l'INSERM, France. durand@cnrs-orleans.fr |
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Abstract: | Using circular dichroism spectroscopy the ability of berenil, a minor groove binding drug, to induce triple helix formation was investigated with two oligonucleotides designed to form two intramolecular triplexes containing T*A:T and G*G:C triplets, which differ only by the orientation of their third strand: 5'-d(G4A4G4-[T4]-C4T4C4-[T4]-G4T4G4), and 5'-d(G4T4G4-[T4]-G4A4G4-[T4]-C4T4C4), where [T4] represents a stretch of four thymine residues. We demonstrate that when added to the duplex form of these oligonucleotides, berenil induces triplex structure formation only if the orientation of third strand is anti-parallel to the purine strand. |
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