Inhibition of cytochrome c oxidase by psychosine (galactosylsphingosine) |
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| Authors: | H Igisu M Nakamura |
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| Affiliation: | 1. Département de Chimie and Centre BioMed, Université du Québec à Montréal, C.P. 8888, Succ. Centre-ville, Montreal, Quebec H3C 3P8, Canada;2. Centre for Research in Neuroscience, The Research Institute of the McGill University Health Center, 1650 Cedar Ave., Montreal, Quebec H3G 1A4, Canada;1. Department of Pediatrics, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan;2. Department of Pediatrics, Taipei Medical University Hospital, Taipei, Taiwan;3. Department of Anatomy and Cellular Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan |
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| Abstract: | Pi uptake and acetoacetate formation were suppressed by psychosine (galactosylsphingosine) in rat liver mitochondria. Besides, reduced form of cytochrome c increased in the reaction mixture which contained psychosine. Using reduced form of cytochrome c as substrate, less than 5 microM of psychosine (0.1 mumoles/mg of mitochondrial protein) inhibited cytochrome c oxidase by more than 50%. The inhibition was completely reversed by 1% human serum albumin. Thus, a lipid which is produced in the brain has a powerful and yet reversible inhibitory effect on an enzyme of cellular respiration. |
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